SUSCEPTIBILITY TO MITOMYCIN C AND LECITHINASE ACTIVITIES OF CLOSTRIDIUM OEDEMATIENS (C. NOVYI) TYPES B AND D

Physiologic Action of Clostridium Oedematiens (novyi) Toxin in Dogs.

Biochemical Properties of the Toxins of Clostridium novyi and Clostridium hemolyticum.

The report of Oakley, Warrack, and Clarke (1947) on the toxins of the Clostridium novyi (oedematiens) group and of Clostridium hemolyticum indicates the complexity of this topic and presents the pertinent historical data. It is known that the toxins C. novyi and C. hemolyticum involve a lecithinase catalyzing the hydrolysis of lecithin to phosphorylcholine and a diglyceride. In view of the exis...

application of triplex pcr technique in identification of clostridium perfringens b, c and d types

in this research 130 sheep-dung samples were collected from kermani breed in area of kerman randomly. after processing and culture of samples, the produced colonies were studied morphologically and gram stain test and the genus of these bacteria were identified using biochemical tests. the clostridia strains isolates were included 30 percent of total samples i.e. clostridiums perfringense, bara...

In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B

Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...

Phospholipase C produced by Clostridium botulinum types C and D: comparison of gene, enzymatic, and biological activities with those of Clostridium perfringens alpha-toxin.

Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were ...